- Serial Vs Parallel Dilution serial#
- Serial Vs Parallel Dilution manual#
- Serial Vs Parallel Dilution portable#
- Serial Vs Parallel Dilution series#
Critical Review: digital resolution biomolecular sensing for diagnostics and life science research. Qinglan Huang, Nantao Li, Hanyuan Zhang, Congnyu Che, Fu Sun, Yanyu Xiong, Taylor D.A fully automated centrifugal microfluidic system for sample-to-answer viral nucleic acid testing.
Fei Tian, Chao Liu, Jinqi Deng, Ziwei Han, Lu Zhang, Qinghua Chen, Jiashu Sun.Slip formation of a high-density droplet array for nucleic acid quantification by digital LAMP with a random-access system. Weiyuan Lyu, Jiajie Zhang, Yan Yu, Lei Xu, Feng Shen.
Serial Vs Parallel Dilution portable#
Portable integrated digital PCR system for the point-of-care quantification of BK virus from urine samples. Lei Xu, Haijun Qu, Daniel Garcia Alonso, Ziqing Yu, Yan Yu, Yejing Shi, Chunlan Hu, Tongyu Zhu, Nannan Wu, Feng Shen.Multiplexed droplet loop-mediated isothermal amplification with scorpion-shaped probes and fluorescence microscopic counting for digital quantification of virus RNAs. Ya-Ling Tan, A-Qian Huang, Li-Juan Tang, Jian-Hui Jiang.Sensors and Actuators B: Chemical 2021, 22, 130493. Establishment of Scalable Nanoliter Digital LAMP Technology for the Quantitative Detection of Multiple Myeloproliferative Neoplasm Molecular Markers. Guojun Cao, Jinze Li, Zhifang Xing, Zhiqi Zhang, Wei Zhang, Chuanyu Li, Longhui Li, Zhen Guo, Shuli Li, Xu Gao, Yanchun Ma, Lianqun Zhou, Ming Guan.Rapid Fabrication by Digital Light Processing 3D Printing of a SlipChip with Movable Ports for Local Delivery to Ex Vivo Organ Cultures. Megan A Catterton, Alexander G Ball, Rebecca R Pompano.Multiplex digital PCR with digital melting curve analysis on a self-partitioning SlipChip. Yan Yu, Ziqing Yu, Xufeng Pan, Lei Xu, Rui Guo, Xiaohua Qian, Feng Shen.TrAC Trends in Analytical Chemistry 2022, 149, 116568. Advances in improvement strategies of digital nucleic acid amplification for pathogen detection.
Single-cell approaches in human microbiome research. Recent Advances in Digital Biosensing Technology. Fike, Brandi Binkley, Toktam Godary, Peng Li. Digital Loop-Mediated Isothermal Amplification-Based Absolute Methylation Quantification Revealed Hypermethylated DAPK1 in Cervical Cancer Patients.
Sensitive, Single-Pot, Two-Stage Assay for Hepatitis Viruses.
Serial Vs Parallel Dilution serial#
With 64 wells of each dilution and fewer than 600 wells in total, the serial dilution SlipChip can achieve a theoretical quantification dynamic range of 7 orders of magnitude. Furthermore, an eight-step serial dilution SlipChip with a dilution ratio of 1:4 is applied for digital loop-mediated isothermal amplification (LAMP) across a large dynamic range and tested for hepatitis B viral load quantification with clinical samples. The dilution ratio can be precisely predetermined by the volumes of mother microwells and daughter microwells, and this paper demonstrates devices designed to have dilution ratios of 1:1, 1:2, and 1:4.
Serial Vs Parallel Dilution series#
Here, a multistep SlipChip microfluidic device is presented for generating serial dilution nanoliter arrays in high throughput with a series of simple sliding motions. Moreover, it is very challenging to set up an array of serial dilutions in nanoliter volumes in miniaturized assays.
Serial Vs Parallel Dilution manual#
Creating an array of serial dilutions usually requires cumbersome manual pipetting steps or a robotic liquid handling system. Serial dilution is a commonly used technique that generates a low-concentration working sample from a high-concentration stock solution and is used to set up screening conditions over a large dynamic range for biological study, optimization of reaction conditions, drug screening, etc.